验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67032 targets NEU1 in IF/ICC applications and shows reactivity with Human, Rat, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Rat, Pig |
| 免疫原 | NEU1 fusion protein Ag27945 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | sialidase 1 (lysosomal sialidase) |
| 别名 | Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | BC011900 |
| 基因名称 | NEU1 |
| Gene ID (NCBI) | 4758 |
| RRID | AB_3084301 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99519 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NEU1 antibody CL488-67032 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |