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验证数据展示

  • IF Staining of mouse brain using CL488-28180

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-28180

    IF Staining of mouse brain using CL488-28180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NET1 antibody (CL488-28180) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using CL488-28180

    FC experiment of Jurkat using CL488-28180

    1x10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NET1 (CL488-28180)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NET1 Polyclonal antibody

NET1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-28180

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

IF-P, FC (Intra)

别名

ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy NET1 Ready-To-Use IHC Kit. NET1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28180-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28180 targets NET1 in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 Human, Mouse
免疫原

CatNo: Ag28142

Product name: Recombinant human NET1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 442-542 aa of BC010285

Sequence: IRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroepithelial cell transforming 1
别名 ARHGEF8, NET1, NET1A, Proto oncogene p65 Net1
计算分子量 596 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC010285
基因名称 NET1
Gene ID (NCBI) 10276
RRIDAB_3672824
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z628
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
IF protocol for CL Plus 488 NET1 antibody CL488-28180Download protocol
Standard Protocols
Click here to view our Standard Protocols
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