验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, mouse brain tissue, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy NET1 Ready-To-Use IHC Kit. NET1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28180-1-AP targets NET1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28142 Product name: Recombinant human NET1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 442-542 aa of BC010285 Sequence: IRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuroepithelial cell transforming 1 |
| 别名 | Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8 |
| 计算分子量 | 596 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC010285 |
| 基因名称 | NET1 |
| Gene ID (NCBI) | 10276 |
| RRID | AB_2881082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z628 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NET1 antibody 28180-1-AP | Download protocol |
| IHC protocol for NET1 antibody 28180-1-AP | Download protocol |
| WB protocol for NET1 antibody 28180-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastric Cancer LncRNA CTC-497E21.4 promotes the progression of gastric cancer via modulating miR-22/NET1 axis through RhoA signaling pathway. | ||
Reprod Biol Endocrinol NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes
| ||
Front Cardiovasc Med Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis. | ||
Gastroenterology Cell-in-Cell-Mediated Entosis Reveals A Progressive Mechanism in Pancreatic Cancer
| ||
iScience Neuroepithelial cell-transforming 1 promotes cardiac fibrosis via the Wnt/β-catenin signaling pathway
| ||
Theriogenology Reduced NET1 adversely affects early embryonic development in mice
|
