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验证数据展示

  • WB analysis using 84880-1-RR

    WB analysis using 84880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84880-1-RR (NDUFS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 84880-1-RR

    IHC staining of human stomach cancer using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 84880-1-RR

    IHC staining of human stomach cancer using 84880-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84880-1-RR (NDUFS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 84880-1-RR

    FC experiment of HepG2 using 84880-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDUFS2 Recombinant antibody (84880-1-RR, Clone:242238D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84880-1-RR

    Affinity and Kinetic Characterization of 84880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84880-1-RR against Human NDUFS2 were performed. The affinity constant is 1.61 nM.

NDUFS2 Recombinant monoclonal antibody

NDUFS2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84880-1-RR

产品说明书

CloneNo. 242238D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

242238D4, CI 49kD, CI-49kD, Complex I 49kD, Complex I-49kD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84880-1-RR targets NDUFS2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28008

Product name: Recombinant human NDUFS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-464 aa of BC000170

Sequence: GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDLGAMTPFFWLFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKNFSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
别名 242238D4, CI 49kD, CI-49kD, Complex I 49kD, Complex I-49kD
计算分子量 53 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC000170
基因名称 NDUFS2
Gene ID (NCBI) 4720
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial Complex I, responsible for the oxidation of NADH, is suspected to be an integral component of the O2-sensing pathway. NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2), a key subunit of complex I in the mitochondrial electron transport chain (ETC), is essential for acute oxygen-sensing and hypoxic pulmonary vasoconstriction (PMID: 30922174). Functions of NDUFS2 are associated with growth, cell-membrane integrity, ROS generation, apoptosis, necrosis, Complex I respiration, and synthesis of ATP (PMID: 33744462).

实验方案

Product Specific Protocols
FC protocol for NDUFS2 antibody 84880-1-RRDownload protocol
IHC protocol for NDUFS2 antibody 84880-1-RRDownload protocol
WB protocol for NDUFS2 antibody 84880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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