验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28125-1-AP targets NDUFS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27078 Product name: Recombinant human NDUFS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 119-464 aa of BC000170 Sequence: GTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDLGAMTPFFWLFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKNFSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) |
| 别名 | CI 49kD, CI-49kD, Complex I 49kD, Complex I-49kD, EC:7.1.1.2 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC000170 |
| 基因名称 | NDUFS2 |
| Gene ID (NCBI) | 4720 |
| RRID | AB_3669645 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75306 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFS2 (NADH:Ubiquinone Oxidoreductase Core Subunit S2) is a crucial nuclear-encoded protein component of mitochondrial complex I (NADH dehydrogenase), the first and largest complex in the mitochondrial electron transport chain. It functions as part of the enzyme's core matrix arm, directly contributing to the oxidation of NADH, the transfer of electrons to ubiquinone, and the coupled translocation of protons across the inner mitochondrial membrane, which is essential for ATP synthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFS2 antibody 28125-1-AP | Download protocol |
| IHC protocol for NDUFS2 antibody 28125-1-AP | Download protocol |
| WB protocol for NDUFS2 antibody 28125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
