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Species Reactivity
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验证数据展示

  • WB analysis using 28125-1-AP

    WB analysis using 28125-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28125-1-AP (NDUFS2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 28125-1-AP

    IF Staining of HepG2 using 28125-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS2 antibody (28125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

NDUFS2 Polyclonal antibody

NDUFS2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28125-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28125-1-AP targets NDUFS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDUFS2 fusion protein Ag27078 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
别名 NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, EC:7.1.1.2, Complex I-49kD, Complex I 49kD, CI-49kD
计算分子量 53 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC000170
基因名称 NDUFS2
Gene ID (NCBI) 4720
RRIDAB_3669645
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFS2 antibody 28125-1-APDownload protocol
IF protocol for NDUFS2 antibody 28125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols