• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated NDUFS1 Monoclonal antibody

NDUFS1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H5E8

Cat No : CL488-68253

Print datasheet

Synonyms

CI 75Kd, Complex I 75kD, NDUFS1, PRO1304

验证数据展示

  • IF Staining of HepG2 using CL488-68253

    IF Staining of HepG2 using CL488-68253

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 NDUFS1 antibody (CL488-68253, Clone: 3H5E8 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-68253

    FC experiment of Jurkat using CL488-68253

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NDUFS1 (CL488-68253, Clone:3H5E8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68253-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68253 targets NDUFS1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen NDUFS1 fusion protein Ag3135 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Synonyms CI 75Kd, Complex I 75kD, NDUFS1, PRO1304
Calculated Molecular Weight 727 aa, 79 kDa
Observed Molecular Weight68-81 kDa
GenBank Accession NumberBC030833
Gene Symbol NDUFS1
Gene ID (NCBI) 4719
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28331
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NDUFS1 antibody CL488-68253Download protocol
FC protocol for CL Plus 488 NDUFS1 antibody CL488-68253Download protocol
Standard Protocols
Click here to view our Standard Protocols