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验证数据展示

  • WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 12444-1-AP (same clone as 12444-1-PBS)

    WB analysis of HEK-293 using 12444-1-AP (same clone as 12444-1-PBS)

    WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    WB analysis using 12444-1-AP (same clone as 12444-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

    IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS1 antibody included
  • IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

    IHC staining of human liver cancer using 12444-1-AP (same clone as 12444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS1 antibody included
  • IF Staining of HepG2 using 12444-1-AP (same clone as 12444-1-PBS)

    IF Staining of HepG2 using 12444-1-AP (same clone as 12444-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 12444-1-AP (same clone as 12444-1-PBS)

    IP experiment of mouse lung using 12444-1-AP (same clone as 12444-1-PBS)

    IP result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 12444-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NDUFS1 Polyclonal antibody, PBS Only

NDUFS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12444-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NDUFS1 Ready-To-Use IHC Kit. NDUFS1 primary antibody included.

产品信息

12444-1-PBS targets NDUFS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3135

Product name: Recombinant human NDUFS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-305 aa of BC030833

Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
别名
计算分子量 727 aa, 79 kDa
观测分子量 74-81 kDa
GenBank蛋白编号BC030833
基因名称 NDUFS1
Gene ID (NCBI) 4719
RRIDAB_2282657
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28331
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

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