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验证数据展示

  • WB analysis using 85141-4-RR

    WB analysis using 85141-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85141-4-RR (NDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 85141-4-RR

    IHC staining of human lung cancer using 85141-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 85141-4-RR

    IP experiment of HeLa using 85141-4-RR

    IP result of anti-NDRG1 (IP:85141-4-RR, 4ug; Detection:85141-4-RR 1:1000) with HeLa cells lysate 1520 ug.

  • IHC staining of human lung cancer using 85141-4-RR

    IHC staining of human lung cancer using 85141-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 85141-4-RR

    FC experiment of HeLa using 85141-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDRG1 Recombinant antibody (85141-4-RR, Clone:242322F7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85141-4-RR

    Affinity and Kinetic Characterization of 85141-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85141-4-RR against Human NDRG1 were performed. The affinity constant is below 1 pM.

NDRG1 Recombinant antibody

NDRG1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 85141-4-RR

产品说明书

CloneNo. 242322F7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, FC (Intra), IP, ELISA

别名

242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, rat kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85141-4-RR targets NDRG1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, rat
免疫原 NDRG1 fusion protein Ag25424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-myc downstream regulated 1
别名 242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1
计算分子量 43 kDa
观测分子量45-46 kDa
GenBank蛋白编号BC006260
基因名称 NDRG1
Gene ID (NCBI) 10397
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92597
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

实验方案

Product Specific Protocols
WB protocol for NDRG1 antibody 85141-4-RRDownload protocol
IHC protocol for NDRG1 antibody 85141-4-RRDownload protocol
IP protocol for NDRG1 antibody 85141-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols