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验证数据展示

  • WB analysis using 85141-4-RR (same clone as 85141-4-PBS)

    WB analysis using 85141-4-RR (same clone as 85141-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85141-4-RR (NDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    IP experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    IP result of anti-NDRG1 (IP:85141-4-RR, 4ug; Detection:85141-4-RR 1:1000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    FC experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDRG1 Recombinant antibody (85141-4-RR, Clone:242322F7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85141-4-RR

    Affinity and Kinetic Characterization of 85141-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85141-4-RR against Human NDRG1 were performed. The affinity constant is below 1 pM.

NDRG1 Recombinant monoclonal antibody, PBS Only

NDRG1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 85141-4-PBS

产品说明书

CloneNo. 242322F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85141-4-PBS targets NDRG1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25424

Product name: Recombinant human NDRG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 45-109 aa of BC006260

Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-myc downstream regulated 1
别名 242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1
计算分子量 43 kDa
观测分子量45-46 kDa
GenBank蛋白编号BC006260
基因名称 NDRG1
Gene ID (NCBI) 10397
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92597
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

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