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验证数据展示

  • WB analysis using CL647-67616

    WB analysis using CL647-67616

    Various lysates were subjected to SDS PAGE followed by western blot with CL647-67616 (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using CL647-67616

    FC experiment of SH-SY5Y using CL647-67616

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human NCS1 (CL647-67616, Clone:2F10C8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 647-conjugated NCS1 Monoclonal antibody

NCS1 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL647-67616

产品说明书

CloneNo. 2F10C8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, FC (Intra)

别名

FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 67616-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67616 targets NCS1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag29808

Product name: Recombinant human FREQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-190 aa of BC004856

Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 frequenin homolog (Drosophila)
别名 FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein
计算分子量 22 kDa
观测分子量 20 kDa
GenBank蛋白编号BC004856
基因名称 NCS1
Gene ID (NCBI) 23413
RRIDAB_2920295
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62166
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL Plus 647 NCS1 antibody CL647-67616Download protocol
Standard Protocols
Click here to view our Standard Protocols
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