NCS1 Monoclonal antibody
NCS1 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2F10C8
验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, U-87 MG cells, pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67616-1-Ig targets NCS1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Immunogen | NCS1 fusion protein Ag29808 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | frequenin homolog (Drosophila) |
| Synonyms | DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC004856 |
| Gene Symbol | FREQ |
| Gene ID (NCBI) | 23413 |
| RRID | AB_2882819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCS1 antibody 67616-1-Ig | Download protocol |
| IHC protocol for NCS1 antibody 67616-1-Ig | Download protocol |
| IF protocol for NCS1 antibody 67616-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons |