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验证数据展示

  • FC experiment of HEK-293 using 85004-1-RR (same clone as 85004-1-PBS)

    FC experiment of HEK-293 using 85004-1-RR (same clone as 85004-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Recombinant antibody (85004-1-RR, Clone:241969G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85004-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 85004-1-RR (same clone as 85004-1-PBS)

    IF Staining of A431 using 85004-1-RR (same clone as 85004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (85004-1-RR, Clone: 241969G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85004-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 85004-1-RR (same clone as 85004-1-PBS)

    IF Staining of A431 using 85004-1-RR (same clone as 85004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (85004-1-RR, Clone: 241969G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85004-1-PBS in a different storage buffer formulation.

NCOA5 Recombinant monoclonal antibody, PBS Only

NCOA5 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85004-1-PBS

产品说明书

CloneNo. 241969G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

241969G3, CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85004-1-PBS targets NCOA5 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14418

Product name: Recombinant human NCOA5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 112-384 aa of BC056872

Sequence: DMRDSRDPMYRREGSYDRYLRMDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRQYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear receptor coactivator 5
别名 241969G3, CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5
计算分子量 579 aa, 66 kDa
GenBank蛋白编号BC056872
基因名称 NCOA5
Gene ID (NCBI) 57727
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HCD5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear receptor coactivator 5 (NCOA5), also known as a coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription and the c-Myc gene expression (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to maintaining the pluripotent stem cell population (PMID: 24268775).

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