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验证数据展示

  • FC experiment of HEK-293 using 85004-1-RR

    FC experiment of HEK-293 using 85004-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Recombinant antibody (85004-1-RR, Clone:241969G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of A431 using 85004-1-RR

    IF Staining of A431 using 85004-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (85004-1-RR, Clone: 241969G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 85004-1-RR

    IF Staining of A431 using 85004-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (85004-1-RR, Clone: 241969G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NCOA5 Recombinant monoclonal antibody

NCOA5 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85004-1-RR

产品说明书

CloneNo. 241969G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

241969G3, CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85004-1-RR targets NCOA5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14418

Product name: Recombinant human NCOA5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 112-384 aa of BC056872

Sequence: DMRDSRDPMYRREGSYDRYLRMDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRQYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear receptor coactivator 5
别名 241969G3, CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5
计算分子量 579 aa, 66 kDa
GenBank蛋白编号BC056872
基因名称 NCOA5
Gene ID (NCBI) 57727
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HCD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor coactivator 5 (NCOA5), also known as a coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription and the c-Myc gene expression (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to maintaining the pluripotent stem cell population (PMID: 24268775).

实验方案

Product Specific Protocols
IF protocol for NCOA5 antibody 85004-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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