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CoraLite® Plus 488-conjugated NCAPG Monoclonal antibody

NCAPG Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4D3

Cat No : CL488-68082

Print datasheet

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

验证数据展示

  • IF Staining of U2OS using CL488-68082

    IF Staining of U2OS using CL488-68082

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 NCAPG antibody (CL488-68082, Clone: 2D4D3 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-68082

    FC experiment of U2OS using CL488-68082

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NCAPG (CL488-68082, Clone:2D4D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68082-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68082 targets NCAPG in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen NCAPG fusion protein Ag19926 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name non-SMC condensin I complex, subunit G
Synonyms CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog
Calculated Molecular Weight 1015 aa, 114 kDa
Observed Molecular Weight114 kDa
GenBank Accession NumberBC068467
Gene Symbol NCAPG
Gene ID (NCBI) 64151
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BPX3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NCAPG antibody CL488-68082Download protocol
FC protocol for CL Plus 488 NCAPG antibody CL488-68082Download protocol
Standard Protocols
Click here to view our Standard Protocols