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验证数据展示

  • IF Staining of MCF-7 using CL488-14551

    IF Staining of MCF-7 using CL488-14551

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 NAC1 antibody (CL488-14551) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated NAC1 Polyclonal antibody

NAC1 Polyclonal Antibody for IF/ICC
Cat No. CL488-14551

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

NACC1, Nucleus accumbens-associated protein 1, NAC-1, BTBD14B, BTB/POZ domain-containing protein 14B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NACC1 Ready-To-Use IHC Kit. NACC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14551-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14551 targets NAC1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 NAC1 fusion protein Ag6096 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
别名 NACC1, Nucleus accumbens-associated protein 1, NAC-1, BTBD14B, BTB/POZ domain-containing protein 14B
计算分子量 57 kDa
观测分子量 60 kDa
GenBank蛋白编号BC055396
基因名称 NACC1
Gene ID (NCBI) 112939
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RE7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NACC1, also named as BTBD14B and NAC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). NACC1 has 1 isoform with the molecular mass of 57 kDa. While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NAC1 antibody CL488-14551Download protocol
Standard Protocols
Click here to view our Standard Protocols