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验证数据展示

  • WB analysis using 85380-2-RR

    WB analysis using 85380-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85380-2-RR (NAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 85380-2-RR

    IF Staining of MCF-7 using 85380-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NAC1 antibody (85380-2-RR, Clone: 242788E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human ovary cancer using 85380-2-RR

    IHC staining of human ovary cancer using 85380-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85380-2-RR (NAC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NACC1 antibody included
  • FC experiment of HeLa using 85380-2-RR

    FC experiment of HeLa using 85380-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NAC1 Recombinant antibody (85380-2-RR, Clone:242788E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85380-2-RR

    Affinity and Kinetic Characterization of 85380-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85380-2-RR against Human NAC1 were performed. The affinity constant is 1.39 nM.

NAC1 Recombinant monoclonal antibody

NAC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 85380-2-RR

产品说明书

CloneNo. 242788E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

NACC1, 242788E11, BEND8, BTB/POZ domain-containing protein 14B, BTBD14B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, SH-SY5Y cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, SH-SY5Y cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NACC1 Ready-To-Use IHC Kit. NACC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85380-2-RR targets NAC1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6096

Product name: Recombinant human NACC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-527 aa of BC055396

Sequence: SDSVQCMPVAKRLWDSGQKEAGGGGNGSRKMAKFSTPDLAANRPHQPPPPQQAPVVAAAQPAVAAGAGQPAGGVAAAGGVVSGPSTSERTSPGTSSAYTSDSPGSYHNEEDEEEDGGEEGMDEQYRQICNMYTMYSMMNVGQTAEKVEALPEQVAPESRNRIRVRQDLASLPAELINQIGNRCHPKLYDEGDPSEKLELVTGTNVYITRAQLMNCHVSAGTRHKVLLRRLLASFFDRNTLANSCGTGIRSSTNDPRRKPLDSRVLHAVKYYCQNFAPNFKESEMNAIAADMCTNARRVVRKSWMPKVKVLKAEDDAYTTFISETGKIEPDMMGVEHGFETASHEGEAGPSAEALQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
别名 NACC1, 242788E11, BEND8, BTB/POZ domain-containing protein 14B, BTBD14B
计算分子量 57 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC055396
基因名称 NACC1
Gene ID (NCBI) 112939
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96RE7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAC1, also named as BTBD14B and NACC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549). The observed molecular weight is consistent with what has been described in the literature (PMID: 37533751).

实验方案

Product Specific Protocols
FC protocol for NAC1 antibody 85380-2-RRDownload protocol
IF protocol for NAC1 antibody 85380-2-RRDownload protocol
IHC protocol for NAC1 antibody 85380-2-RRDownload protocol
WB protocol for NAC1 antibody 85380-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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