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验证数据展示

  • WB analysis of HeLa using 32235-1-AP (same clone as 32235-1-PBS)

    WB analysis of HeLa using 32235-1-AP (same clone as 32235-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

    IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NACA antibody included
  • IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

    IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NACA antibody included

NACA Polyclonal antibody, PBS Only

NACA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32235-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NACA Ready-To-Use IHC Kit. NACA primary antibody included.

产品信息

32235-1-PBS targets NACA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37704

Product name: Recombinant human NACA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-215 aa of BC106041

Sequence: MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nascent polypeptide-associated complex alpha subunit
别名 Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1
计算分子量 215 aa, 23 kDa
观测分子量35 kDa
GenBank蛋白编号BC106041
基因名称 NACA
Gene ID (NCBI) 4666
RRIDAB_3670227
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13765
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).

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