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验证数据展示

  • WB analysis of HeLa using 32235-1-AP

    WB analysis of HeLa using 32235-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 32235-1-AP

    IHC staining of mouse kidney using 32235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 32235-1-AP

    IHC staining of mouse kidney using 32235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NACA Polyclonal antibody

NACA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32235-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32235-1-AP targets NACA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NACA fusion protein Ag37704 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nascent polypeptide-associated complex alpha subunit
别名 Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1
计算分子量 215 aa, 23 kDa
观测分子量35 kDa
GenBank蛋白编号BC106041
基因名称 NACA
Gene ID (NCBI) 4666
RRIDAB_3670227
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).

实验方案

Product Specific Protocols
WB protocol for NACA antibody 32235-1-APDownload protocol
IHC protocol for NACA antibody 32235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols