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验证数据展示

  • FC experiment of MCF-7 using CL750-83178-2

    FC experiment of MCF-7 using CL750-83178-2

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 N4bp3 Recombinant Antibody (CL750-83178-2, Clone:230547D12)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using CL750-83178-2

    WB analysis using CL750-83178-2

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83178-2 (N4BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated N4BP3 Recombinant antibody

N4BP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83178-2

产品说明书

CloneNo. 230547D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra)

别名

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 83178-2-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83178-2 targets N4BP3 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 N4BP3 fusion protein Ag10194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Nedd4 binding protein 3
别名 NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12
计算分子量 544 aa, 60 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC053323
基因名称 N4BP3
Gene ID (NCBI) 23138
RRIDAB_3673776
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15049
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 N4BP3 antibody CL750-83178-2Download protocol
FC protocol for CL Plus 750 N4BP3 antibody CL750-83178-2Download protocol
Standard Protocols
Click here to view our Standard Protocols