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验证数据展示

  • FC experiment of MCF-7 using 83178-2-RR

    FC experiment of MCF-7 using 83178-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug N4BP3 Recombinant antibody (83178-2-RR, Clone:230547D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of MCF-7 using 83178-2-RR

    WB analysis of MCF-7 using 83178-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83178-2-RR (N4BP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 83178-2-RR

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 83178-2-RR

    IHC staining of human liver using 83178-2-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 83178-2-RR (N4BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83178-2-RR

    Affinity and Kinetic Characterization of 83178-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83178-2-RR against Human N4BP3 were performed. The affinity constant is 66.3 pM.

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N4BP3 Recombinant monoclonal antibody

N4BP3 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83178-2-RR

产品说明书

CloneNo. 230547D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83178-2-RR targets N4BP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10194

Product name: Recombinant human N4BP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 198-544 aa of BC053323

Sequence: GRSPGTGPSPFSSSLGHLNHLGGSLDRASQGPKEAGPPAVLSCLPEPPPPYEFSCSSAEEMGAVLPETCEELKRGLGDEDGSNPFTQVLEERQRLWLAELKRLYVERLHEVTQKAERSERNLQLQLFMAQQEQRRLRKELRAQQGLAPEPRAPGTLPEADPSARPEEEARWEVCQKTAEISLLKQQLREAQAELAQKLAEIFSLKTQLRGSRAQAQAQDAELVRLREAVRSLQEQAPREEAPGSCETDDCKSRGLLGEAGGSEARDSAEQLRAELLQERLRGQEQALRFEQERRTWQEEKERVLRYQREIQGGYMDMYRRNQALEQELRALREPPTPWSPRLESSKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Nedd4 binding protein 3
别名 NEDD4-binding protein 3, Nedd4 binding protein 3, KIAA0341, 230547D12
计算分子量 544 aa, 60 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC053323
基因名称 N4BP3
Gene ID (NCBI) 23138
RRIDAB_3670871
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15049
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons.

实验方案

Product Specific Protocols
WB protocol for N4BP3 antibody 83178-2-RRDownload protocol
IHC protocol for N4BP3 antibody 83178-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols