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验证数据展示

  • IF Staining of C2C12 using CL488-66205

    IF Staining of C2C12 using CL488-66205

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Myoglobin antibody (CL488-66205, Clone: 1A4F7 ) at dilution of 1:200.

  • FC experiment of C2C12 using CL488-66205

    FC experiment of C2C12 using CL488-66205

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Myoglobin (CL488-66205, Clone:1A4F7) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Myoglobin Monoclonal antibody

Myoglobin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66205

产品说明书

CloneNo. 1A4F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 66205-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66205 targets Myoglobin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8979

Product name: Recombinant human MB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-154 aa of BC014547

Sequence: MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myoglobin
别名 MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB
计算分子量 154 aa, 17 kDa
观测分子量 17-18 kDa
GenBank蛋白编号BC014547
基因名称 Myoglobin
Gene ID (NCBI) 4151
RRIDAB_3084221
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02144
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Myoglobin antibody CL488-66205Download protocol
Standard Protocols
Click here to view our Standard Protocols