验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs, A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 66360-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66360-1 targets IgG1 Isotype Control in IF, FC applications and shows reactivity with megathura crenulata samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | megathura crenulata |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IgG1 Isotype Control |
| 别名 | Mouse IgG1 isotype control, Kappa light chain, Mouse IgG1 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2934458 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IgG1 Isotype Control antibody CL488-66360-1 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |