Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata and More (1)

Applications

FC, WB, ELISA and More (5)

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat No : 66360-1-Ig

Print datasheet

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control



经过测试的应用

Positive WB detected in
Positive FC detected in

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-1-Ig targets Mouse IgG1 isotype control in WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Applications FC, WB, ELISA
Cited Applications Cell treatment, CoIP, IHC, IP, RIP, WB
Tested Reactivity Megathura crenulata
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Mouse IgG1 isotype control
Synonyms Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2827991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
FC protocol for Mouse IgG1 isotype control antibody 66360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
CoIP

FASEB J

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection

Authors - Chujie Ding
IP

Exp Cell Res

CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.

Authors - Yuting Yang
IHC

Am J Rhinol Allergy

Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.

Authors - Tomohiro Kawasumi
RIP

Cell Biosci

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

Authors - Liang Chen

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li
IP

Acta Biochim Biophys Sin (Shanghai)

PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation

Authors - Gongyu Fu