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验证数据展示

  • IF Staining of HeLa using CL488-68142

    IF Staining of HeLa using CL488-68142

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 MYL6 antibody (CL488-68142, Clone: 2G4C9 ) at dilution of 1:500.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MYL6 Monoclonal antibody

MYL6 Monoclonal Antibody for IF/ICC
Cat No. CL488-68142

产品说明书

CloneNo. 2G4C9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

IF/ICC

别名

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68142-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68142 targets MYL6 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 MYL6 fusion protein Ag13091 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 6, alkali, smooth muscle and non-muscle
别名 17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6
计算分子量 17 kDa
观测分子量17-25 kDa
GenBank蛋白编号BC006781
基因名称 MYL6
Gene ID (NCBI) 4637
RRIDAB_3084415
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60660
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, 2304459, 2722814).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYL6 antibody CL488-68142Download protocol
Standard Protocols
Click here to view our Standard Protocols