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  • Featured Product
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CoraLite® Plus 488-conjugated MYL6 Monoclonal antibody

MYL6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G4C9

Cat No : CL488-68142

Print datasheet

Synonyms

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

验证数据展示

  • IF Staining of HeLa using CL488-68142

    IF Staining of HeLa using CL488-68142

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 MYL6 antibody (CL488-68142, Clone: 2G4C9 ) at dilution of 1:500.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68142-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68142 targets MYL6 in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen MYL6 fusion protein Ag13091 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 6, alkali, smooth muscle and non-muscle
Synonyms 17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17-25 kDa
GenBank Accession NumberBC006781
Gene Symbol MYL6
Gene ID (NCBI) 4637
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60660
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, 2304459, 2722814).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYL6 antibody CL488-68142Download protocol
Standard Protocols
Click here to view our Standard Protocols