验证数据展示
经过测试的应用
| Positive WB detected in | pig heart tissue, A549 cells, rabbit heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human colon tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human kidney tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
68142-1-Ig targets MYL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 | MYL6 fusion protein Ag13091 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 6, alkali, smooth muscle and non-muscle |
| 别名 | 17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17-25 kDa |
| GenBank蛋白编号 | BC006781 |
| 基因名称 | MYL6 |
| Gene ID (NCBI) | 4637 |
| RRID | AB_2935258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P60660 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL6 antibody 68142-1-Ig | Download protocol |
| IHC protocol for MYL6 antibody 68142-1-Ig | Download protocol |
| IF protocol for MYL6 antibody 68142-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol Disulfidptosis-related gene expression reflects the prognosis of drug-resistant cancer patients and inhibition of MYH9 reverses sorafenib resistance | ||
Int J Biol Macromol Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways |
