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验证数据展示

  • WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51759-1

    Cytometric bead array sample test of MP51759-1

    Cytometric bead array sample test of MP51759-1, MYL6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68142-1-PBS. Detection antibody: 68142-2-PBS.

  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51759-1

    Cytometric bead array standard curve of MP51759-1

    Cytometric bead array standard curve of MP51759-1, MYL6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68142-1-PBS. Detection antibody: 68142-2-PBS. Standard:Ag13091. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO Validated

MYL6 Monoclonal antibody, PBS Only (Capture)

MYL6 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68142-1-PBS

产品说明书

CloneNo. 2G4C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

2G4C9, MLC-3, MYL 6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.

产品信息

68142-1-PBS targets MYL6 as part of a matched antibody pair:

MP51759-1: 68142-1-PBS capture and 68142-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag13091

Product name: Recombinant human MYL6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-151 aa of BC006781

Sequence: MCDFTEDQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVAGHEDSNGCINYEAFVRHILSG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 6, alkali, smooth muscle and non-muscle
别名 2G4C9, MLC-3, MYL 6
计算分子量 17 kDa
观测分子量17-25 kDa
GenBank蛋白编号BC006781
基因名称 MYL6
Gene ID (NCBI) 4637
RRIDAB_2935258
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP60660
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).

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