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验证数据展示

  • WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYL6 Monoclonal antibody, PBS Only

MYL6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68142-1-PBS

产品说明书

CloneNo. 2G4C9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.

产品信息

68142-1-PBS targets MYL6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 MYL6 fusion protein Ag13091 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 6, alkali, smooth muscle and non-muscle
别名 17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6
计算分子量 17 kDa
观测分子量17-25 kDa
GenBank蛋白编号BC006781
基因名称 MYL6
Gene ID (NCBI) 4637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60660
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).