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0
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0
Conjugate
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验证数据展示

  • WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

    WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67533-1-Ig (same clone as 67533-1-PBS)

    IHC staining of human heart using 67533-1-Ig (same clone as 67533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 67533-1-Ig (same clone as 67533-1-PBS)

    IF Staining of mouse heart using 67533-1-Ig (same clone as 67533-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

  • WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

    WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 67533-1-Ig (same clone as 67533-1-PBS)

    FC experiment of C2C12 using 67533-1-Ig (same clone as 67533-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYL4 Monoclonal antibody, PBS Only

MYL4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 67533-1-PBS

产品说明书

CloneNo. 2F5C1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

Myosin light chain alkali GT-1 isoform, Myosin light chain 4, MYL 4, MLC1, 2F5C1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67533-1-PBS targets MYL4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 MYL4 fusion protein Ag19244 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 4, alkali; atrial, embryonic
别名 Myosin light chain alkali GT-1 isoform, Myosin light chain 4, MYL 4, MLC1, 2F5C1
计算分子量 197 aa, 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC030228
基因名称 MYL4
Gene ID (NCBI) 4635
RRIDAB_2882752
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12829
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.