验证数据展示
经过测试的应用
| Positive WB detected in | pig heart tissue, K-562 cells, Mo7e cells, TF-1 cells, rat heart tissues, mouse heart tissues |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67533-1-Ig targets MYL4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | MYL4 fusion protein Ag19244 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 4, alkali; atrial, embryonic |
| 别名 | Myosin light chain alkali GT-1 isoform, Myosin light chain 4, MYL 4, MLC1, 2F5C1 |
| 计算分子量 | 197 aa, 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC030228 |
| 基因名称 | MYL4 |
| Gene ID (NCBI) | 4635 |
| RRID | AB_2882752 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P12829 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| IHC protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| IF protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| FC protocol for MYL4 antibody 67533-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes. | ||
J Proteome Res Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts | ||
Genes (Basel) Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs
| ||
Gene Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size | ||
Cell Biosci Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers | ||
Front Med Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart |