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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11128

Print datasheet

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9

验证数据展示

  • IF Staining of HepG2 using CL488-11128

    IF Staining of HepG2 using CL488-11128

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MYH9 antibody (CL488-11128) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11128-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11128 targets MYH9 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen MYH9 fusion protein Ag1612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 9, non-muscle
Synonyms DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 224 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35579
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYH9 antibody CL488-11128Download protocol
Standard Protocols
Click here to view our Standard Protocols