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验证数据展示

  • WB analysis using 11128-1-AP

    WB analysis using 11128-1-AP

    Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11128-1-AP

    IHC staining of human lung cancer using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH9 antibody included
  • IHC staining of human lung cancer using 11128-1-AP

    IHC staining of human lung cancer using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH9 antibody included
  • IP experiment of mouse brain using 11128-1-AP

    IP experiment of mouse brain using 11128-1-AP

    IP result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human colon using 11128-1-AP

    IHC staining of human colon using 11128-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11128-1-AP (MYH9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH9 antibody included
  • IF Staining of mouse small intestine using 11128-1-AP

    IF Staining of mouse small intestine using 11128-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 11128-1-AP

    IF Staining of HepG2 using 11128-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of U2OS using 11128-1-AP

    IF Staining of U2OS using 11128-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MYH9 antibody (11128-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 11128-1-AP

    FC experiment of HepG2 using 11128-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYH9 Polyclonal antibody (11128-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 11128-1-AP

产品说明书

引用文献(82)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)

别名

Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9, NMMHC II-a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, mouse kidney tissue, mouse colon tissue, human brain tissue, U2OS cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue
Positive IF/ICC detected inHepG2 cells, U2OS cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11128-1-AP targets MYH9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken
免疫原

CatNo: Ag1612

Product name: Recombinant human MYH9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1741-1960 aa of BC011915

Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 9, non-muscle
别名 Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9, NMMHC II-a
计算分子量 227 kDa
观测分子量 224 kDa
GenBank蛋白编号BC011915
基因名称 MYH9
Gene ID (NCBI) 4627
RRIDAB_2147294
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35579
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

实验方案

Product Specific Protocols
FC protocol for MYH9 antibody 11128-1-APDownload protocol
WB protocol for MYH9 antibody 11128-1-APDownload protocol
IP protocol for MYH9 antibody 11128-1-APDownload protocol
IHC protocol for MYH9 antibody 11128-1-APDownload protocol
IF protocol for MYH9 antibody 11128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

The p-MYH9/USP22/HIF-1α axis promotes lenvatinib resistance and cancer stemness in hepatocellular carcinoma

Authors - Qiaonan Shan
View Article
humanWB,IP,IF

Nature

TMEFF1 is a neuron-specific restriction factor for herpes simplex virus

Authors - Yao Dai
  • KO Validated
View Article
humanWB,CoIP

Mol Cancer

OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway

Authors - Hongfa Wei
View Article
IF

Nat Biomed Eng

PIEZO1 mechanically regulates the antitumour cytotoxicity of T lymphocytes

Authors - Ruiyang Pang
View Article
mouseWB

Nat Commun

Cell state dependent effects of Bmal1 on melanoma immunity and tumorigenicity

Authors - Xue Zhang
View Article
humanWB

Nat Commun

Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation

Authors - Siyuan Sun
View Article
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