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验证数据展示

  • WB analysis of COLO 320 using 66825-1-Ig (same clone as 66825-1-PBS)

    WB analysis of COLO 320 using 66825-1-Ig (same clone as 66825-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH14 antibody included
  • IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH14 antibody included
  • IF Staining of mouse skeletal muscle using 66825-1-Ig (same clone as 66825-1-PBS)

    IF Staining of mouse skeletal muscle using 66825-1-Ig (same clone as 66825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MYH14 Monoclonal antibody, PBS Only

MYH14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66825-1-PBS

产品说明书

CloneNo. 1B4C9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

myosin, MHC16, KIAA2034, FP17425, DFNA4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYH14 Ready-To-Use IHC Kit. MYH14 primary antibody included.

产品信息

66825-1-PBS targets MYH14 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28063

Product name: Recombinant human MYH14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1231-1430 aa of NM_001077186

Sequence: MQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 14
别名 myosin, MHC16, KIAA2034, FP17425, DFNA4
计算分子量 228 kDa
观测分子量 200-230 kDa
GenBank蛋白编号NM_001077186
基因名称 MYH14
Gene ID (NCBI) 79784
RRIDAB_2882168
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z406
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

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