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验证数据展示

  • WB analysis of COLO 320 using 66825-1-Ig

    WB analysis of COLO 320 using 66825-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66825-1-Ig

    IHC staining of human colon using 66825-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH14 antibody included
  • IHC staining of human colon using 66825-1-Ig

    IHC staining of human colon using 66825-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH14 antibody included
  • IF Staining of mouse skeletal muscle using 66825-1-Ig

    IF Staining of mouse skeletal muscle using 66825-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

MYH14 Monoclonal antibody

MYH14 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66825-1-Ig

产品说明书

CloneNo. 1B4C9
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HT-29 cells
Positive IHC detected inhuman colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MYH14 Ready-To-Use IHC Kit. MYH14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66825-1-Ig targets MYH14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, mouse
文献引用反应性rat
免疫原 MYH14 fusion protein Ag28063 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 14
别名 DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C
计算分子量 228 kDa
观测分子量 200-230 kDa
GenBank蛋白编号NM_001077186
基因名称 MYH14
Gene ID (NCBI) 79784
RRIDAB_2882168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z406
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

实验方案

Product Specific Protocols
WB protocol for MYH14 antibody 66825-1-IgDownload protocol
IHC protocol for MYH14 antibody 66825-1-IgDownload protocol
IF protocol for MYH14 antibody 66825-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

J Cell Sci

Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation

Authors - Helena Sofia Domingues
  • KD Validated
View Article
IF

J Cell Sci

Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.

Authors - Helena Sofia Domingues
View Article