验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HT-29 cells |
| Positive IHC detected in | human colon tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66825-1-Ig targets MYH14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag28063 Product name: Recombinant human MYH14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1231-1430 aa of NM_001077186 Sequence: MQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy chain 14 |
| 别名 | DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C |
| 计算分子量 | 228 kDa |
| 观测分子量 | 200-230 kDa |
| GenBank蛋白编号 | NM_001077186 |
| 基因名称 | MYH14 |
| Gene ID (NCBI) | 79784 |
| RRID | AB_2882168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z406 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| IHC protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| WB protocol for MYH14 antibody 66825-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation
| ||
J Cell Sci Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation. |
