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验证数据展示

  • IF Staining of HepG2 using CL488-68131

    IF Staining of HepG2 using CL488-68131

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MYDGF/C19orf10 antibody (CL488-68131, Clone: 1H8F4 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-68131

    FC experiment of HepG2 using CL488-68131

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MYDGF/C19orf10 (CL488-68131, Clone:1H8F4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated MYDGF/C19orf10 Monoclonal antibody

MYDGF/C19orf10 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68131

产品说明书

CloneNo. 1H8F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYDGF Ready-To-Use IHC Kit. MYDGF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68131-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68131 targets MYDGF/C19orf10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MYDGF/C19orf10 fusion protein Ag13438 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 19 open reading frame 10
别名 MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor
计算分子量 173 aa, 19 kDa
观测分子量15-19 kDa
GenBank蛋白编号BC010129
基因名称 MYDGF/C19orf10
Gene ID (NCBI) 56005
RRIDAB_3084412
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ969H8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYDGF/C19orf10 antibody CL488-68131Download protocol
FC protocol for CL Plus 488 MYDGF/C19orf10 antibody CL488-68131Download protocol
Standard Protocols
Click here to view our Standard Protocols