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验证数据展示

  • WB analysis using CL594-67608

    WB analysis using CL594-67608

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67608 (MYBPC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

CoraLite®594-conjugated MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for WB
Cat No. CL594-67608

产品说明书

CloneNo. 3C8F8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB

别名

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 67608-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67608 targets MYBPC3 in WB applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB Application Description
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag15848

Product name: Recombinant human MYBPC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-328 aa of BC151211

Sequence: MPEPGKKPVSAFSKKPRSVEVAAGSPAVFEAETERAGVKVRWQRGGSDISASNKYGLATEGTRHTLTVREVGPADQGSYAVIAGSSKVKFDLKVIEAEKAEPMLAPAPAPAEATGAPGEAPAPAAELGESAPSPKGSSSAALNGPTPGAPDDPIGLFVMRPQDGEVTVGGSITFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYLFELHITDAQPAFTGSYRCEVSTKDKFDCSNFNLTVHEAMGTGDLDLLSAFRRTSLAGGGRRISDSHEDTGILDFSSLLKKRDSFRTPRDSKLEAPAEEDVWEILRQA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin binding protein C, cardiac
别名 Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3
计算分子量 1274 aa, 141 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC151211
基因名称 MYBPC3
Gene ID (NCBI) 4607
RRIDAB_3673521
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14896
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

实验方案

Product Specific Protocols
WB protocol for CL594 MYBPC3 antibody CL594-67608Download protocol
Standard Protocols
Click here to view our Standard Protocols
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