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验证数据展示

  • IHC staining of human tonsillitis using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human tonsillitis using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colon using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human colon using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colon cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human colon cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colon cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human colon cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human appendicitis using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human appendicitis using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human rectal cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    IHC staining of human rectal cancer using 66172-2-Ig (same clone as 66172-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • Cytometric bead array standard curve of MP50291-1

    Cytometric bead array standard curve of MP50291-1

    Cytometric bead array standard curve of MP50291-1, MSH6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66172-2-PBS. Detection antibody: 66172-3-PBS. Standard:Ag12645. Range: 3.125-200 ng/mL.

  • Cytometric bead array standard curve of MP50291-2

    Cytometric bead array standard curve of MP50291-2

    Cytometric bead array standard curve of MP50291-2, MSH6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66172-2-PBS. Detection antibody: 66172-4-PBS. Standard:Ag12645. Range: 6.25-200 ng/mL.

MSH6 Monoclonal antibody, PBS Only (Capture)

MSH6 Monoclonal Antibody for IHC, Cytometric bead array, Indirect ELISA
Cat No. 66172-2-PBS

产品说明书

CloneNo. 5B5A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, Cytometric bead array, Indirect ELISA

别名

5B5A7, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.

产品信息

66172-2-PBS targets MSH6 as part of a matched antibody pair:

MP50291-1: 66172-2-PBS capture and 66172-3-PBS detection (validated in Cytometric bead array)

MP50291-2: 66172-2-PBS capture and 66172-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12645

Product name: Recombinant human MSH6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC004246

Sequence: MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 6 (E. coli)
别名 5B5A7, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP
计算分子量 153 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC004246
基因名称 MSH6
Gene ID (NCBI) 2956
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP52701
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

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