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验证数据展示

  • IHC staining of human tonsillitis using 66172-2-Ig

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colon cancer using 66172-2-Ig

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colon using 66172-2-Ig

    IHC staining of human colon using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human appendicitis using 66172-2-Ig

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human rectal cancer using 66172-2-Ig

    IHC staining of human rectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human appendicitis using 66172-2-Ig

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66172-2-Ig

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 66172-2-Ig

    IHC staining of human ovary cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 66172-2-Ig

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colon cancer using 66172-2-Ig

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colorectal cancer using 66172-2-Ig

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human colorectal cancer using 66172-2-Ig

    IHC staining of human colorectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human ovary cancer using 66172-2-Ig

    IHC staining of human ovary cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human stomach cancer using 66172-2-Ig

    IHC staining of human stomach cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human appendicitis using 66172-2-Ig

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included
  • IHC staining of human tonsillitis using 66172-2-Ig

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH6 antibody included

MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for IHC, IHC-Autostainer, ELISA
Cat No. 66172-2-Ig

产品说明书

CloneNo. 5B5A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IHC-Autostainer, ELISA

别名

5B5A7, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC-Autostainer detected inhuman tonsillitis tissue, human stomach cancer tissue, human colorectal cancer tissue, human ovary cancer tissue, human appendicitis tissue
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human colorectal cancer tissue, human colon cancer tissue, human ovary cancer tissue, human stomach cancer tissue, human rectal cancer tissue, human tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66172-2-Ig targets MSH6 in IHC, IHC-Autostainer, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IHC-Autostainer, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12645

Product name: Recombinant human MSH6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC004246

Sequence: MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 6 (E. coli)
别名 5B5A7, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP
计算分子量 153 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC004246
基因名称 MSH6
Gene ID (NCBI) 2956
RRIDAB_3743303
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP52701
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

实验方案

Product Specific Protocols
IHC protocol for MSH6 antibody 66172-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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