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验证数据展示

  • WB analysis using 83515-4-RR (same clone as 83515-4-PBS)

    WB analysis using 83515-4-RR (same clone as 83515-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83515-4-RR (MRPL40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR (same clone as 83515-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83515-4-RR (same clone as 83515-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83515-4-RR (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83515-4-RR (same clone as 83515-4-PBS)

    IF Staining of HeLa using 83515-4-RR (same clone as 83515-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL40 antibody (83515-4-RR, Clone: 240506A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83515-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83515-4-RR

    Affinity and Kinetic Characterization of 83515-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83515-4-RR against Human MRPL40 were performed. The affinity constant is 1.56 nM.

MRPL40 Recombinant antibody, PBS Only

MRPL40 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83515-4-PBS

产品说明书

CloneNo. 240506A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83515-4-PBS targets MRPL40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRPL40 fusion protein Ag10341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial ribosomal protein L40
别名 MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40, L40mt
计算分子量 206 aa, 24 kDa
观测分子量18-24 kDa
GenBank蛋白编号BC009707
基因名称 MRPL40
Gene ID (NCBI) 64976
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NQ50
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MRPL40, also known as NLVCF and URIM, belongs to the mitochondrion-specific ribosomal protein mL40 family. MRPL40 encodes the mitochondrial ribosomal protein L40 for the larger 39S subunit of mitochondrial ribosomes. MRPL40 is widely expressed in human organs and tissues, including the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon, peripheral blood lymphocyte (PBL), and testis. MRPL40 is also expressed in human fetuses and mouse embryos, suggesting its important role in fetal development (PMID: 36891938).