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验证数据展示

  • WB analysis using 85070-5-RR (same clone as 85070-5-PBS)

    WB analysis using 85070-5-RR (same clone as 85070-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85070-5-RR (MRPL12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85070-5-RR (same clone as 85070-5-PBS)

    IHC staining of human colon cancer using 85070-5-RR (same clone as 85070-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85070-5-RR (MRPL12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MRPL12 antibody included
  • IHC staining of mouse stomach using 85070-5-RR (same clone as 85070-5-PBS)

    IHC staining of mouse stomach using 85070-5-RR (same clone as 85070-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85070-5-RR (MRPL12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MRPL12 antibody included
  • IF Staining of HepG2 using 85070-5-RR (same clone as 85070-5-PBS)

    IF Staining of HepG2 using 85070-5-RR (same clone as 85070-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (85070-5-RR, Clone: 242622D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85070-5-RR

    Affinity and Kinetic Characterization of 85070-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85070-5-RR against Human MRPL12 were performed. The affinity constant is below 1 pM.

MRPL12 Recombinant monoclonal antibody, PBS Only

MRPL12 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85070-5-PBS

产品说明书

CloneNo. 242622D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242622D12, 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MRPL12 Ready-To-Use IHC Kit. MRPL12 primary antibody included.

产品信息

85070-5-PBS targets MRPL12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6504

Product name: Recombinant human MRPL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC002344

Sequence: MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial ribosomal protein L12
别名 242622D12, 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC002344
基因名称 MRPL12
Gene ID (NCBI) 6182
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52815
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.

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