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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MPO Monoclonal antibody

MPO Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgA

Reactivity

human, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C11F6

Cat No : CL488-66177

Print datasheet

Synonyms

MPO, myeloperoxidase

验证数据展示

  • IF Staining of human tonsillitis using CL488-66177

    IF Staining of human tonsillitis using CL488-66177

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 MPO antibody (CL488-66177, Clone: 4C11F6 ) at dilution of 1:200.

  • FC experiment of HL-60 using CL488-66177

    FC experiment of HL-60 using CL488-66177

    1X10^6 HL-60 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MPO (CL488-66177, Clone:4C11F6) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHL-60 cells

Not recommend for IHC about rat sample. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66177-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66177 targets MPO in IF, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, rat
Immunogen MPO fusion protein Ag17564 种属同源性预测
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Full Name myeloperoxidase
Synonyms MPO, myeloperoxidase
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
RRIDAB_2919295
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP05164
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MPO antibody CL488-66177Download protocol
FC protocol for CL Plus 488 MPO antibody CL488-66177Download protocol
Standard Protocols
Click here to view our Standard Protocols