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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MOV10 Polyclonal antibody

MOV10 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10370

Print datasheet

Synonyms

DKFZp667O1423, FLJ32791, fSAP113, gb110, KIAA1631, MOV10, Putative helicase MOV 10

验证数据展示

  • IF Staining of HepG2 using CL488-10370

    IF Staining of HepG2 using CL488-10370

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MOV10 antibody (CL488-10370) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10370

    FC experiment of HeLa using CL488-10370

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MOV10 (CL488-10370) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MOV10 Ready-To-Use IHC Kit. MOV10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10370-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10370 targets MOV10 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen MOV10 fusion protein Ag0384 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mov10, Moloney leukemia virus 10, homolog (mouse)
Synonyms DKFZp667O1423, FLJ32791, fSAP113, gb110, KIAA1631, MOV10, Putative helicase MOV 10
Calculated Molecular Weight 114 kDa
Observed Molecular Weight 110-115 kDa
GenBank Accession NumberBC002548
Gene Symbol MOV10
Gene ID (NCBI) 4343
RRIDAB_2918975
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCE1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MOV10 antibody CL488-10370Download protocol
FC protocol for CL Plus 488 MOV10 antibody CL488-10370Download protocol
Standard Protocols
Click here to view our Standard Protocols