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验证数据展示

  • WB analysis using 83398-1-RR (same clone as 83398-1-PBS)

    WB analysis using 83398-1-RR (same clone as 83398-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83398-1-RR (same clone as 83398-1-PBS)

    WB analysis of HeLa using 83398-1-RR (same clone as 83398-1-PBS)

    WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83398-1-RR (same clone as 83398-1-PBS)

    IHC staining of mouse brain using 83398-1-RR (same clone as 83398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MOSPD2 antibody included
  • IHC staining of mouse placenta using 83398-1-RR (same clone as 83398-1-PBS)

    IHC staining of mouse placenta using 83398-1-RR (same clone as 83398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MOSPD2 antibody included
  • FC experiment of A431 using 83398-1-RR (same clone as 83398-1-PBS)

    FC experiment of A431 using 83398-1-RR (same clone as 83398-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00419-2

    Cytometric bead array standard curve of MP00419-2

    Cytometric bead array standard curve of MP00419-2, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-1-PBS. Detection antibody: 83398-2-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00419-3

    Cytometric bead array standard curve of MP00419-3

    Cytometric bead array standard curve of MP00419-3, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-3-PBS. Detection antibody: 83398-1-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83398-1-RR

    Affinity and Kinetic Characterization of 83398-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

  • Featured Product
  • KD/KO验证

MOSPD2 Recombinant antibody, PBS Only (Capture/Detector)

MOSPD2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83398-1-PBS

产品说明书

CloneNo. 240282C11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240282C11, Motile sperm domain-containing protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MOSPD2 Ready-To-Use IHC Kit. MOSPD2 primary antibody included.

产品信息

83398-1-PBS targets MOSPD2 as part of a matched antibody pair:

MP00419-2: 83398-1-PBS capture and 83398-2-PBS detection (validated in Cytometric bead array)

MP00419-3: 83398-3-PBS capture and 83398-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 MOSPD2 fusion protein Ag22206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 motile sperm domain containing 2
别名 240282C11, Motile sperm domain-containing protein 2
计算分子量 518 aa, 60 kDa
观测分子量 59 kDa
GenBank蛋白编号BC030641
基因名称 MOSPD2
Gene ID (NCBI) 158747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NHP6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).