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验证数据展示

  • WB analysis using 85211-4-RR (same clone as 85211-4-PBS)

    WB analysis using 85211-4-RR (same clone as 85211-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85211-4-RR (MMP-8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85211-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85211-4-RR (same clone as 85211-4-PBS)

    IHC staining of mouse spleen using 85211-4-RR (same clone as 85211-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85211-4-RR (MMP8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85211-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85211-4-RR (same clone as 85211-4-PBS)

    IHC staining of mouse spleen using 85211-4-RR (same clone as 85211-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85211-4-RR (MMP8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85211-4-PBS in a different storage buffer formulation.

Rat MMP8 Recombinant monoclonal antibody, PBS Only

MMP8 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85211-4-PBS

产品说明书

CloneNo. 242473H8

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, Indirect ELISA

别名

EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85211-4-PBS targets MMP8 in WB, IHC, Indirect ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 rat
免疫原

CatNo: Eg3278

Product name: Recombinant Rat MMP-8 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 21-466 aa of NM_022221.1

Sequence: LPVPPEHLEEKNMKTAENYLRKFYHLPSNQFRSARNATMIAEKLKEMQRFFGLPETGKPDAATIEIMEKPRCGVPDSGDFLLTPGSPKWTHTNLTYRIINHTPQMSKAEVKTEIEKAFKIWSVPSTLTFTETLEGEADINIAFVSRDHGDNSPFDGPNGILAHAFQPGRGIGGDAHFDSEETWTQDSKNYNLFLVAAHEFGHSLGLSHSTDPGALMYPNYAYREPSTYSLPQDDINGIQTIYGPSDNPVQPTGPSTPTACDPHLRFDAATTLRGEIYFFKDKYFWRRHPQLRTVDLNFISLFWPFLPNGLQAAYEDFDRDLVFLFKGRQYWALSAYDLQQGYPRDISNYGFPRSVQAIDAAVSYNGKTYFFVNNQCWRYDNQRRSMDPGYPTSIASVFPGINCRIDAVFQQDSFFLFFSGPQYFAFNLVSRRVTRVARSNLWLNCP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 8
别名 EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase
计算分子量53 kDa
观测分子量 60-70 kDa
GenBank蛋白编号NM_022221.1
基因名称 Mmp8
Gene ID (NCBI) 63849
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO88766
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).

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