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验证数据展示

  • WB analysis using 82984-4-RR

    WB analysis using 82984-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 82984-4-RR

    FC experiment of HepG2 using 82984-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82984-4-RR

    Affinity and Kinetic Characterization of 82984-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

MMP3 Recombinant antibody

MMP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82984-4-RR

产品说明书

CloneNo. 230535G8
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, FC (Intra), ELISA and More (1)

别名

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-87 MG cells, A2780 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82984-4-RR targets MMP3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 3 (stromelysin 1, progelatinase)
别名 SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17
观测分子量55 kDa
GenBank蛋白编号BC074815
基因名称 MMP3
Gene ID (NCBI) 4314
RRIDAB_3670734
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08254
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases.

实验方案

Product Specific Protocols
WB protocol for MMP3 antibody 82984-4-RRDownload protocol
FC protocol for MMP3 antibody 82984-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Bone Joint Res

Inhibition of PA28γ expression can alleviate osteoarthritis by inhibiting endoplasmic reticulum stress and promoting STAT3 phosphorylation

Authors - Haokun Mo
View Article