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验证数据展示

  • WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

    WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

    FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82984-4-RR

    Affinity and Kinetic Characterization of 82984-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

MMP3 Recombinant monoclonal antibody, PBS Only

MMP3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82984-4-PBS

产品说明书

CloneNo. 230535G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

230535G8, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.

产品信息

82984-4-PBS targets MMP3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0382

Product name: Recombinant Human MMP-3 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 18-477 aa of BC074815

Sequence: YPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 3 (stromelysin 1, progelatinase)
别名 230535G8, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3
GenBank蛋白编号BC074815
基因名称 MMP3
Gene ID (NCBI) 4314
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08254
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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