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验证数据展示

  • Sandwich ELISA standard curve of MP00290-1

    Sandwich ELISA standard curve of MP00290-1

    Sandwich ELISA standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. 83114-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0527. 83114-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    Plasma of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 653.4 pg/mL with a range of 99.3 - 1,631.8 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    HT1080 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human MMP1, and measured 907.5 pg/mL.

  • Sample test of MP00290-1

    Sample test of MP00290-1

    Serum of fourteen individual healthy human donors were measured. The human MMP1 concentration of detected samples was determined to be 9,124.3 pg/mL with a range of 3,045.5 - 19,864.3 pg/mL.

  • Cytometric bead array standard curve of MP00290-1

    Cytometric bead array standard curve of MP00290-1

    Cytometric bead array standard curve of MP00290-1, MMP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83114-4-PBS. Detection antibody: 83114-3-PBS. Standard: Eg0527. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83114-3-PBS

    Affinity and Kinetic Characterization of 83114-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83114-3-PBS against Human MMP1 was performed. The affinity constant is 12.7 nM.

MMP1 Recombinant monoclonal antibody, PBS Only (Detector)

MMP1 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83114-3-PBS

产品说明书

CloneNo. 230343B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMP1 Ready-To-Use IHC Kit. MMP1 primary antibody included.

产品信息

83114-3-PBS targets MMP1 as part of a matched antibody pair:

MP00290-1: 83114-4-PBS capture and 83114-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Eg0527

Product name: Recombinant human MMP1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 20-469 aa of BC013875

Sequence: FPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 1 (interstitial collagenase)
别名 22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7
计算分子量 54 kDa
GenBank蛋白编号BC013875
基因名称 MMP1
Gene ID (NCBI) 4312
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP03956
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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