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验证数据展示

  • WB analysis using 84903-1-RR

    WB analysis using 84903-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84903-1-RR

    Affinity and Kinetic Characterization of 84903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

MKL1/MRTF-A Recombinant antibody

MKL1/MRTF-A Recombinant Antibody for WB, ELISA
Cat No. 84903-1-RR

产品说明书

CloneNo. 242021B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MRTFA/MKL1 Ready-To-Use IHC Kit. MRTFA/MKL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84903-1-RR targets MKL1/MRTF-A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MKL1/MRTF-A fusion protein Ag15308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 megakaryoblastic leukemia (translocation) 1
别名 MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438
计算分子量 931 aa, 99 kDa
观测分子量145-160 kDa
GenBank蛋白编号BC115039
基因名称 MKL1
Gene ID (NCBI) 57591
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ969V6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).

实验方案

Product Specific Protocols
WB protocol for MKL1/MRTF-A antibody 84903-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols