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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84903-1-RR (same clone as 84903-1-PBS)

    WB analysis using 84903-1-RR (same clone as 84903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84903-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84903-1-RR

    Affinity and Kinetic Characterization of 84903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

MKL1/MRTF-A Recombinant antibody, PBS Only

MKL1/MRTF-A Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84903-1-PBS

产品说明书

CloneNo. 242021B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MRTFA/MKL1 Ready-To-Use IHC Kit. MRTFA/MKL1 primary antibody included.

产品信息

84903-1-PBS targets MKL1/MRTF-A in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 MKL1/MRTF-A fusion protein Ag15308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 megakaryoblastic leukemia (translocation) 1
别名 MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438
计算分子量 931 aa, 99 kDa
观测分子量145-160 kDa
GenBank蛋白编号BC115039
基因名称 MKL1
Gene ID (NCBI) 57591
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ969V6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).