×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 19820-1-AP (same clone as 19820-1-PBS)

    WB analysis using 19820-1-AP (same clone as 19820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19820-1-AP (same clone as 19820-1-PBS)

    IF Staining of HeLa using 19820-1-AP (same clone as 19820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MIS12 antibody (19820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

    IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

    IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MIS12 antibody included

MIS12 Polyclonal antibody, PBS Only

MIS12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19820-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hMis12, KNTC2AP, MTW1, Protein MIS12 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MIS12 Ready-To-Use IHC Kit. MIS12 primary antibody included.

产品信息

19820-1-PBS targets MIS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13870

Product name: Recombinant human MIS12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC000229

Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MIS12, MIND kinetochore complex component, homolog (yeast)
别名 hMis12, KNTC2AP, MTW1, Protein MIS12 homolog
计算分子量 205 aa, 24 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC000229
基因名称 MIS12
Gene ID (NCBI) 79003
RRIDAB_3669315
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H081
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

||
New chat

Able™

正在加载，请稍候...