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验证数据展示

  • WB analysis using 19820-1-AP

    WB analysis using 19820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 19820-1-AP

    IF Staining of HeLa using 19820-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MIS12 antibody (19820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of mouse kidney using 19820-1-AP

    IHC staining of mouse kidney using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of human ovary cancer using 19820-1-AP

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of human placenta using 19820-1-AP

    IHC staining of human placenta using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of mouse ovary using 19820-1-AP

    IHC staining of mouse ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of mouse small intestine using 19820-1-AP

    IHC staining of mouse small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of rat kidney using 19820-1-AP

    IHC staining of rat kidney using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of rat ovary using 19820-1-AP

    IHC staining of rat ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of rat small intestine using 19820-1-AP

    IHC staining of rat small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of mouse ovary using 19820-1-AP

    IHC staining of mouse ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of rat ovary using 19820-1-AP

    IHC staining of rat ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of rat small intestine using 19820-1-AP

    IHC staining of rat small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of human ovary cancer using 19820-1-AP

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included
  • IHC staining of human ovary cancer using 19820-1-AP

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIS12 antibody included

MIS12 Polyclonal antibody

MIS12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19820-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

hMis12, KNTC2AP, MTW1, Protein MIS12 homolog

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue, mouse ovary tissue, rat small intestine tissue, human placenta tissue, mouse small intestine tissue, rat kidney tissue, rat ovary tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MIS12 Ready-To-Use IHC Kit. MIS12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19820-1-AP targets MIS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13870

Product name: Recombinant human MIS12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC000229

Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MIS12, MIND kinetochore complex component, homolog (yeast)
别名 hMis12, KNTC2AP, MTW1, Protein MIS12 homolog
计算分子量 205 aa, 24 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC000229
基因名称 MIS12
Gene ID (NCBI) 79003
RRIDAB_3669315
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

实验方案

Product Specific Protocols
WB protocol for MIS12 antibody 19820-1-APDownload protocol
IHC protocol for MIS12 antibody 19820-1-APDownload protocol
IF protocol for MIS12 antibody 19820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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